Antibacterial and antibiotic-potentiation activities of methanol extracts from Blighia sapida K. D. Koenig (Sapindaceae) against Gram-negative multidrug-resistant bacteria overexpressing efflux pumps

-Ancela W. B. Yendze1, Larissa Mpude; Eric Cadet; Derick J. Assonfack; Valaire Y. Matieta; Jenifer R. N. Kuete; Junior F. Megaptche; Idrios N. Bonsou; Michael F. Kengne; Armelle T. Mbaveng; and Victor Kuete

Abstract Background: The emergence and spread of multidrug-resistant (MDR) Gram-negative bacteria, particularly those overexpressing efflux pumps, make treatment difficult using conventional antibiotics. This study aims to assess the antibacterial activity of methanol extracts (botanicals) from the leaves (BSL) and bark (BSB) of Blighia sapida against a panel of MDR bacteria that overexpress efflux pumps. Methods: Phytochemical screening of botanicals was carried out using standard qualit...

Unveiling the anti-Klebsiella activity of methanol extracts from Hallea ciliata leaves and barks against multidrug-resistant strains overexpressing AcrAB-TolC efflux pumps

-Derick J. Assonfack; Larissa Mpude; Eric Cadet; Ancela W. B. Yendze; Valaire Y. Matieta; Jenifer R. N. Kuete; Junior F. Megaptche; Idrios N. Bonsou; Michael F. Kengne, Victor Kuete; Armelle T. Mbaveng

Abstract Background: Infectious diseases are considered one of the most critical threats to public health. The present work aimed to determine the anti-Klebsiella activity of methanol extracts (botanicals) of Hallea ciliata leaves (HCL) and bark (HCB) against multidrug-resistant phenotypes over-expressing ACrAB-TolC efflux pumps. Methods: The antibacterial activity of botanicals alone and in combination with PAβN and antibiotics, was determined using the broth microdilution method. The ef...

Antibacterial activity and antibiotic-potentiating effects of methanol extracts from Ocimum basilicum and Sarcocephalus latifolius against multidrug-resistant Gram-negative bacteria overexpressing efflux pumps

-Eric Cadet; Derick J. Assonfack; Ancela W. B. Yendze; Larissa Mpude; Valaire Y. Matieta; Jenifer R. N. Kuete; Junior F. Megaptche; Idrios N. Bonsou; Victor Kuete; Armelle T. Mbaveng.

Abstract Background: The inappropriate use of antibiotics against bacterial infections leads to increased bacterial drug resistance. In the present study, the effectiveness of methanol extracts from the leaves and flowers of Ocimum basilicum and the roots of Sarcocephalus latifolius against multidrug-resistant (MDR) Gram-negative bacteria overexpressing efflux pumps was assessed. Methods: The antibacterial properties of crude extracts (botanical, with and without phenylalanine arginine β-...

Antibacterial activity of Sarcocephalus latifolius and Acacia sieberiana, and the effect of their association with antibiotics against multidrug-resistant Staphylococcus aureus

-Larissa Mpude; Ancela W. B. Yendze; Derick J. Assonfack; Eric Cadet; Valaire Y. Matieta; Junior F. Megaptche; Armelle T. Mbaveng; Victor Kuete

Abstract Background: Infectious diseases continue to wreak havoc around the world causing directly or indirectly 17 million deaths per year. The present study was carried out to evaluate the in vitro antibacterial activity of the methanol extracts from the root of Sarcocephalus latifolius and from the leaves of Acacia siberiana, and their ability to potentiate the action of antibiotics against multidrug-resistant (MDR) Staphylococcus aureus. Methods: The antibacterial assays were performe...