Catechin Derivatives from Parkia biglobosa Displayed Selective Cytotoxicity Towards Leukemia CCRF-CEM Cell Line and its P-Glycoprotein Expressing Subline CEM/ADR5000

Victor Kuete; Viviane R. Sipowo Tala; Armelle T. Mbaveng; Viviane Cândida da Silva; Clenilson M. Rodrigues; Augustin E. Nkengfack; Lourdes Campaner dos Santos; Wagner Vilegas; Thomas Efferth. | E-mail: kuetevictor@yahoo.fr | Received: | Accepted: | Published: 2018-04-26

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Investigational Medicinal Chemistry and Pharmacology 2018; 1(1):2

Abstract

Background: Cancer chemotherapy is challenged by drug resistance of malignant cells. In the present work, we evaluated the cytotoxicity of four catechin derivatives, 4’-methylepigallocatechin (1), gallocatechin (2), epigallocatechin (3), epigallocatechin-3-O-gallate (4) against nine drug sensitive and multidrug resistant (MDR) cancer cell lines.

Methods: The resazurin reduction assay was used to evaluate the cytotoxicity of the compounds.

Results: Catechin derivatives 1-4 displayed selective cytotoxic effect with IC50 values below 90 µM on the leukemia CCRF-CEM cells and it drug resistant subline CEM/ADR5000. In contrast, no IC50 values could be measured in all 7 carcinoma cell lines tested. CEM/ADR5000 cells were much more cross-resistant to doxorubicin than to compounds 1-4.

Conclusions: Hence, the four compounds may serve as lead drugs for further derivatization and can be explored in more details for their possible use to treat leukemia.

 

Keywords: catechin; cytotoxicity; epigallocatechin-3-O-gallate; multidrug resistance; leukemia.

 

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